NSJ Bioreagents

KRT19 Antibody / Cytokeratin 19

Product Code:
 
NSJ-V2680
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
Ks19.1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the KRT19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Immunofluorescent staining of MeOH fixed human MCF7 cells with KRT19 antibody (clone Ks19.1, green) and Reddot nuclear stain (red).~
2 / 2
IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with KRT19 Ab (clone Ks19.1).

Immunofluorescent staining of MeOH fixed human MCF7 cells with KRT19 antibody (clone Ks19.1, green) and Reddot nuclear stain (red).~
IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with KRT19 Ab (clone Ks19.1).

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CodeSizePrice
NSJ-V2680-20UG20 ug£264.00
Quantity:
NSJ-V2680-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the KRT19 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This mAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kDa. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human breast cancer MCF-7 cells were used as the immunogen for the KRT19 antibody.
Limitation:
This KRT19 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P08727