NSJ Bioreagents

KRT10 Antibody / Cytokeratin 10

Product Code:
 
NSJ-V2174
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LH2
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Cytokeratin 10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Western blot testing of human thymus lysate with Cytokeratin 10 antibody. Predicted molecular weight ~59 kDa.
2 / 3
IHC testing of human bladder carcinoma with Keratin 10 antibody (clone LH2).
3 / 3
SDS-PAGE analysis of purified, BSA-free Cytokeratin 10 antibody (clone LH2) as confirmation of integrity and purity.

Western blot testing of human thymus lysate with Cytokeratin 10 antibody. Predicted molecular weight ~59 kDa.
IHC testing of human bladder carcinoma with Keratin 10 antibody (clone LH2).
SDS-PAGE analysis of purified, BSA-free Cytokeratin 10 antibody (clone LH2) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V2174-20UG20 ug£264.00
Quantity:
NSJ-V2174-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). cytokeratin 10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of cytokeratin 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, cytokeratin 10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), cytokeratin 10 is detected very frequently. Expression of cytokeratin 10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
3858
Immunogen:
Skin extract from a human psoriasis patient was used as the immunogen for this Cytokeratin 10 antibody.
Limitation:
This Cytokeratin 10 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human, Mouse