NSJ Bioreagents

Beta Catenin Antibody

Product Code:
 
NSJ-V8478
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rCTNNB1/1507
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant Catenin beta antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Catenin beta antibody (clone rCTNNB1/1507); Red=isotype control, Blue= recombinant Catenin beta antibody.
2 / 3
IHC staining of FFPE human tonsil with recombinant Catenin beta antibody (clone rCTNNB1/1507). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Catenin beta antibody (clone rCTNNB1/1507) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of PFA-fixed human HeLa cells with recombinant Catenin beta antibody (clone rCTNNB1/1507); Red=isotype control, Blue= recombinant Catenin beta antibody.
IHC staining of FFPE human tonsil with recombinant Catenin beta antibody (clone rCTNNB1/1507). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Catenin beta antibody (clone rCTNNB1/1507) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8478-20UG20 ug£264.00
Quantity:
NSJ-V8478-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 0.5-1ug/million cells,ELISA: order Ab without BSA for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant Catenin beta antibody should be determined by the researcher.
Description:
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human beta-Catenin (p120) protein fragment from the human protein was used as the immunogen for the recombinant Catenin beta antibody.
Limitation:
This recombinant Catenin beta antibody is available for research use only.
Localization:
Cell surface and cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P35222