NSJ Bioreagents

Thymidylate Synthase Antibody

Product Code:
 
NSJ-V8144
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rTYMS/1884
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
Storage:
 
Store the recombinant Thymidylate Synthase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant Thymidylate Synthase antibody (clone rTYMS/1884); Red=isotype control, Blue= recombinant Thymidylate Synthase antibody.
2 / 3
Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant Thymidylate Synthase antibody (green, clone rTYMS/1884) and Phalloidin (red).
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Thymidylate Synthase antibody (clone rTYMS/1884) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of PFA-fixed human MOLT4 cells with recombinant Thymidylate Synthase antibody (clone rTYMS/1884); Red=isotype control, Blue= recombinant Thymidylate Synthase antibody.
Immunofluorescent staining of PFA-fixed human MOLT4 cells with recombinant Thymidylate Synthase antibody (green, clone rTYMS/1884) and Phalloidin (red).
SDS-PAGE analysis of purified, BSA-free recombinant Thymidylate Synthase antibody (clone rTYMS/1884) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8144-20UG20 ug£264.00
Quantity:
NSJ-V8144-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Format :
Purified
Formulation :
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A recombinant human partial protein (amino acids 60-174) was used as the immunogen for the recombinant Thymidylate Synthase antibody.
Limitation:
This recombinant Thymidylate Synthase antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P04818