NSJ Bioreagents

MTAP Antibody

Product Code:
 
NSJ-V7321
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
SMTP-1R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant MTAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human HeLa cell lysate with recombinant MTAP antibody (clone SMTP-1R). Expected molecular weight: 26-38 kDa (multiple isoforms).~
2 / 2
IHC staining of FFPE human kidney with recombinant MTAP antibody (clone SMTP-1R). R
❮❯

Western blot testing of human HeLa cell lysate with recombinant MTAP antibody (clone SMTP-1R). Expected molecular weight: 26-38 kDa (multiple isoforms).~
IHC staining of FFPE human kidney with recombinant MTAP antibody (clone SMTP-1R). R

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CodeSizePrice
NSJ-V7321-20UG20 ug£264.00
Quantity:
NSJ-V7321-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
Application Note:
The stated application concentrations are suggested starting points. Titration of the recombinant MTAP antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 97-196 from the human protein was used as the immunogen for the recombinant MTAP antibody.
Limitation:
This recombinant MTAP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human
Uniprot #:
Q13126