Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot the alpha Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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This product comes from:
United States.
Typical lead time:
10-14 working days.
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Further Information
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
The stated application concentrations are suggested starting amounts. Titration of the alpha Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with a-actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an a-actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Antigen affinity purified
In 1X PBS, pH 7.4, with 0.09% sodium azide
A portion of amino acids 346-375 from human ACTA1 was used as the immunogen for this alpha Actin antibody.
This alpha Actin antibody is available for research use only.
Antigen affinity
Human
Species Reactivity (Predicted):
Mouse, Rat, Bovine, Pig, Chicken, Rabbit, Xenopus
P68133