NSJ Bioreagents

PDGFR beta Antibody (CD140b)

Product Code:
 
NSJ-F50659
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store at 4oC for up to one month. For long term, aliquot the PDGFR beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Flow cytometric analysis of SK-Br-3 cells using PDGFR beta antibody (bottom histogram) compared to a?<a href=../search_result.php?search_txt=n1001>negative control</a>?(top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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Confocal immunofluorescent analysis of PDGFR beta antibody with WiDr cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
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IHC analysis of FFPE human breast carcinoma stained with PDGFR beta antibody
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Western blot analysis of anti-PDGFR beta antibody
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Flow cytometric analysis of SK-Br-3 cells using PDGFR beta antibody (bottom histogram) compared to a?<a href=../search_result.php?search_txt=n1001>negative control</a>?(top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of PDGFR beta antibody with WiDr cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
IHC analysis of FFPE human breast carcinoma stained with PDGFR beta antibody
Western blot analysis of anti-PDGFR beta antibody

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CodeSizePrice
NSJ-F50659-0.08ML0.08 ml£260.00
Quantity:
NSJ-F50659-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50,Western blot: 1:1000
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PDGFR beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PDGFRB is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses the gene to that of the translocation, ETS, leukemia gene, results in chronic myelomonocytic leukemia.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 40-72 from the human protein was used as the immunogen for this CD140b / PDGFR beta antibody.
Limitation:
This PDGFR beta antibody is available for research use only.
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P09619