NSJ Bioreagents

RUNX3 Antibody

Product Code:
 
NSJ-F44057
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the RUNX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Western blot analysis of lysate from 1) HL-60 and 2) Jurkat cell line using RUNX3 antibody at 1:1000.
2 / 3
RUNX3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue.
3 / 3
Fluorescent confocal image of HeLa cells stained with RUNX3 antibody. Alexa Fluor 488 secondary was used (green). RUNX3 immunoreactivity is localized to the nucleus and cytoplasm.

Western blot analysis of lysate from 1) HL-60 and 2) Jurkat cell line using RUNX3 antibody at 1:1000.
RUNX3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue.
Fluorescent confocal image of HeLa cells stained with RUNX3 antibody. Alexa Fluor 488 secondary was used (green). RUNX3 immunoreactivity is localized to the nucleus and cytoplasm.

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CodeSizePrice
NSJ-F44057-0.08ML0.08 ml£260.00
Quantity:
NSJ-F44057-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the RUNX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 168-197 from the human protein was used as the immunogen for this RUNX3 antibody.
Limitation:
This RUNX3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q13761