NSJ Bioreagents

CYP1A2 Antibody

Product Code:
 
NSJ-F41377
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot the CYP1A2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
Confocal immunofluorescent analysis of CYP1A2 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
2 / 3
CYP1A2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue.
3 / 3
CYP1A2 antibody western blot analysis in mouse liver tissue lysate. Predicted molecular weight ~58 kDa.
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Confocal immunofluorescent analysis of CYP1A2 antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
CYP1A2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue.
CYP1A2 antibody western blot analysis in mouse liver tissue lysate. Predicted molecular weight ~58 kDa.

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CodeSizePrice
NSJ-F41377-0.08ML0.08 ml£260.00
Quantity:
NSJ-F41377-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50
Application Note:
Titration of the CYP1A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin. [UniProt]
Format :
Antigen affinity purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 255-282 from the human protein was used as the immunogen for this CYP1A2 antibody.
Limitation:
This CYP1A2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse
Uniprot #:
P05177