NSJ Bioreagents

RBM3 Antibody / RNA binding motif protein 3

Product Code:
 
NSJ-F54443
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the RBM3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 5
IHC testing of FFPE human breast carcinoma tissue with RBM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
2 / 5
Western blot testing of human Jurkat cell lysate with RBM3 antibody. Predicted molecular weight ~17 kDa.
3 / 5
Western blot testing of mouse NIH 3T3 cell lysate with RBM3 antibody. Predicted molecular weight ~17 kDa.
4 / 5
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with RBM3 antibody.
5 / 5
Flow cytometry testing of human MCF7 cells with RBM3 antibody; Blue=isotype control, Green= RBM3 antibody.
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IHC testing of FFPE human breast carcinoma tissue with RBM3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human Jurkat cell lysate with RBM3 antibody. Predicted molecular weight ~17 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with RBM3 antibody. Predicted molecular weight ~17 kDa.
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with RBM3 antibody.
Flow cytometry testing of human MCF7 cells with RBM3 antibody; Blue=isotype control, Green= RBM3 antibody.

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CodeSizePrice
NSJ-F54443-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54443-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000,Flow cytometry: 1:25 (1x10e6 cells),Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the RBM3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
RBM3 is a member of the glycine-rich RNA-binding protein family and a protein with one RNA recognition motif (RRM) domain. Expression of this protein is induced by cold shock and low oxygen tension.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 55-84 from the human protein were used as the immunogen for the RBM3 antibody.
Limitation:
This RBM3 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
SAS precipitation
Species Reactivity :
Human
Uniprot #:
P98179