NSJ Bioreagents

KiSS-1 Antibody

Product Code:
 
NSJ-F54765
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the KiSS-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HL60 and 2) Jurkat cell lysate with KiSS-1 antibody. Predicted molecular weight ~15 kDa, commonly observed at 15-20 kDa.
2 / 4
Western blot testing of human 1) placenta and 2) skeletal muscle tissue lysate with KiSS-1 antibody. Predicted molecular weight ~15 kDa, commonly observed at 15-20 kDa.
3 / 4
Western blot testing of human SW480 cell lysate with KiSS-1 antibody. Predicted molecular weight ~15 kDa, commonly observed at 15-20 kDa.
4 / 4
IHC testing of FFPE human testis tissue with KiSS-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of human 1) HL60 and 2) Jurkat cell lysate with KiSS-1 antibody. Predicted molecular weight ~15 kDa, commonly observed at 15-20 kDa.
Western blot testing of human 1) placenta and 2) skeletal muscle tissue lysate with KiSS-1 antibody. Predicted molecular weight ~15 kDa, commonly observed at 15-20 kDa.
Western blot testing of human SW480 cell lysate with KiSS-1 antibody. Predicted molecular weight ~15 kDa, commonly observed at 15-20 kDa.
IHC testing of FFPE human testis tissue with KiSS-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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CodeSizePrice
NSJ-F54765-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54765-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:2000, Immunohistochemistry (FFPE): 1:25
Application Note:
The stated application concentrations are suggested starting points. Titration of the KiSS-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell- matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine- tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 20-52 from the human protein was used as the immunogen for the KiSS-1 antibody.
Limitation:
This KiSS-1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q15726