NSJ Bioreagents

Caveolin-3 Antibody / CAV3

Product Code:
 
NSJ-F54853
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Caveolin-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human HEK293 cell lysate with Caveolin-3 antibody. Predicted molecular weight: ~17 kDa but routinely observed at 20~25 kDa.
2 / 4
Western blot testing of human uterus tissue lysate with Caveolin-3 antibody. Predicted molecular weight: ~17 kDa but routinely observed at 20~25 kDa.
3 / 4
IHC testing of FFPE human skeletal muscle tissue with Caveolin-3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HEK293 cells with Caveolin-3 antibody; Blue=isotype control, Green= Caveolin-3 antibody.
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Western blot testing of human HEK293 cell lysate with Caveolin-3 antibody. Predicted molecular weight: ~17 kDa but routinely observed at 20~25 kDa.
Western blot testing of human uterus tissue lysate with Caveolin-3 antibody. Predicted molecular weight: ~17 kDa but routinely observed at 20~25 kDa.
IHC testing of FFPE human skeletal muscle tissue with Caveolin-3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HEK293 cells with Caveolin-3 antibody; Blue=isotype control, Green= Caveolin-3 antibody.

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CodeSizePrice
NSJ-F54853-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54853-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Western blot: 1:500-1:1000, Immunohistochemistry (FFPE): 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)
Application Note:
The stated application concentrations are suggested starting points. Titration of the Caveolin-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CAV3 is a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in its gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD).
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 5-31 from the human protein was used as the immunogen for the Caveolin-3 antibody.
Limitation:
This Caveolin-3 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Purified
Species Reactivity :
Human
Uniprot #:
P56539