NSJ Bioreagents

Menin Antibody / MEN1

Product Code:
 
NSJ-RQ5651
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Menin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human MCF7 cells with Menin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human HL-60 cells with Menin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Menin antibody.
3 / 3
Western blot testing of 1) human HepG-2, 2) K562, 3) rat PC-12, 4) mouse brain and 5) mouse RAW264.7 lysate with Menin antibody. Predicted molecular weight ~68 kDa.
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Immunofluorescent staining of FFPE human MCF7 cells with Menin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HL-60 cells with Menin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Menin antibody.
Western blot testing of 1) human HepG-2, 2) K562, 3) rat PC-12, 4) mouse brain and 5) mouse RAW264.7 lysate with Menin antibody. Predicted molecular weight ~68 kDa.

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CodeSizePrice
NSJ-RQ5651-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Menin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Menin is a protein that in humans is encoded by the MEN1 gene. It is mapped to 11q13.1. This gene encodes menin, a tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. Menin is a scaffold protein that functions in histone modification and epigenetic gene regulation. It is thought to regulate several pathways and processes by altering chromatin structure through the modification of histones.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids M1-L615) was used as the immunogen for the Menin antibody.
Limitation:
This Menin antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O00255