ELK Biotechnology

Human C4d(Complement Component 4d) ELISA Kit

Product Code:
 
ELK6431
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK6431-48T48T£298.00
Quantity:
ELK6431-96T96T£390.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
C4d
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
2.19-140 ng/mL
Sample Type:
serum, plasma and other biological fluids
Sensitivity:
0.92 ng/mL
Standard:
140 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4d. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4d. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4d, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human C4d in the samples is then determined by comparing the OD of the samples to the standard curve.