ELK Biotechnology

Human PLA2G2D(Phospholipase A2, Group IID) ELISA Kit

Product Code:
 
ELK1754
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK1754-48T48T£298.00
Quantity:
ELK1754-96T96T£390.00
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This product comes from: China.
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Further Information

Alternative Names:
PLA2G2D; sPLA2S; s-PLA2; sPLA2; SPLASH; Secreted Phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2D; Secretory-type PLA, stroma-associated homolog
Assay Length:
3.5h
Assay Type:
Sandwich
Detection Range:
0.32-20 ng/mL
Sample Type:
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity:
0.135 ng/mL
Standard:
20 ng/mL
Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLA2G2D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLA2G2D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLA2G2D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Human PLA2G2D in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID:
Q9UNK4