NSJ Bioreagents

SRP72 Antibody

Product Code:
 
NSJ-F54900
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the SRP72 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
Western blot testing of human 1) HeLa and 2) K562 cell lysate with SRP72 antibody. Predicted molecular weight: 68-75 kDa.
2 / 4
Immunofluorescent staining of human HeLa cells with SRP72 antibody (green) and propidium iodide (nuclear, red).
3 / 4
IHC testing of FFPE human pancreas tissue with SRP72 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 4
Flow cytometry testing of human HeLa cells with SRP72 antibody; Blue=isotype control, Green= SRP72 antibody.
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Western blot testing of human 1) HeLa and 2) K562 cell lysate with SRP72 antibody. Predicted molecular weight: 68-75 kDa.
Immunofluorescent staining of human HeLa cells with SRP72 antibody (green) and propidium iodide (nuclear, red).
IHC testing of FFPE human pancreas tissue with SRP72 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human HeLa cells with SRP72 antibody; Blue=isotype control, Green= SRP72 antibody.

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CodeSizePrice
NSJ-F54900-0.08ML0.08 ml£260.00
Quantity:
NSJ-F54900-0.4ML0.4 ml£502.00
Quantity:
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Further Information

Application Details :
Immunofluorescence: 1:25, Immunohistochemistry (FFPE): 1:25, Flow cytometry: 1:25 (1x10e6 cells), Western blot: 1:500-1:2000
Description:
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. It binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
Format :
Purified
Formulation :
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 119-148 from the human protein was used as the immunogen for the SRP72 antibody.
Limitation:
This SRP72 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
O76094