ProSci

Fibrin beta-chain Antibody [8E5], Rabbit IgG

Product Code:
 
PSI-24-525
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
8E5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Imaging
  • In Vivo Assay
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow-cytometry using the anti-Fibrin beta-chain antibody 8E5 (</b> HepG2 cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r-chimeric version of 8E5 (24-525- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> Flow-cytometry using the anti-Fibrin beta-chain antibody 8E5 (</b> HepG2 cells were fixed using 2% PFA- permeusing 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r-chimeric version of 8E5 (24-525- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

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PSI-24-525-0.2mg0.2mg£681.00
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Further Information

Additional Names:
Factor Ia, factor-1a, fibrin, fibrin ? chain, fibrin beta-chain, fibrin beta chain, fibrin ? chain, Fibrinopeptide B
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic β-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
NCBI Gene ID #:
2244
NCBI Official Name:
HGNC:HGNC:3662
NCBI Official Symbol:
FGB
NCBI Organism:
Homo sapiens
Research Area:
Other
Swissprot #:
P02675
User NOte:
For research use only.