ProSci

TIGIT Antibody [4D4], Rabbit IgG

Product Code:
 
PSI-24-576
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
4D4
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Agonism
  • Flow Cytometry
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b>ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. </b> Binding curves of the rmeric IgG version of the anti-TIGIT antibody 4D4 (24-576; red line) and isotype control (3.0- anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.17 ng/ml was performed using 24-576. For signal detection- a 1:4000 dilution of HRP-lnti-r1 (BioRad 5196-2504) antibody was used. The second increase in e at 1000ng/ml is suggestive of a possible secondary binding epitope for 24-576 on Pr00163-1.9.
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<b>ELISA of anti-TIGIT antibody on TIGIT-Fc fusion protein. </b> Binding curves of the rmeric IgG version of the anti-TIGIT antibody 4D4 (24-576; red line) and isotype control (3.0- anti-Fluorescein; blue line) to an ELISA plate coated with mouse TIGIT-Fc fusion protein (Pr00163-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10-000 to 0.17 ng/ml was performed using 24-576. For signal detection- a 1:4000 dilution of HRP-lnti-r1 (BioRad 5196-2504) antibody was used. The second increase in e at 1000ng/ml is suggestive of a possible secondary binding epitope for 24-576 on Pr00163-1.9.

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PSI-24-576-0.2mg0.2mg£681.00
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Further Information

Additional Names:
VSIG9, T Cell Immunoreceptor With Ig And ITIM Domains, V-Set And Immunoglobulin Domain Containing Protein 9, V-Set And Transmembrane Domain Containing Protein 3, VSTM3, T Cell Immunoreceptor With Ig And ITIM Domains, Washington University Cell Adhesion Molecule, WUCAM
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
Research Area:
Cancer, Immunology, Neuroscience
Swissprot #:
P86176
User NOte:
For research use only.