JC-10

In normal cells, JC-10 selectively aggregated in the mitochondrial matrix to form a reversible red fluorescent polymer (Ex=540 nm, Em=590 nm).In unhealthy mitochondria, due to the decrease or loss of membrane potential, JC-10 is transformed from a polymer into a monomer in the cytoplasm, producing green fluorescence (Ex=490 nm, Em=525 nm).Jc-10 can not only be used for qualitative detection, but also can directly reflect the change of mitochondrial membrane potential due to the change of color.It can also be used for quantitative detection, as the degree of depolarization of mitochondria can be measured by the ratio of red/green fluorescence intensity.The change of these two colors can be detected by the standard filter on flow cytometer. The green fluorescence can be analyzed by FL1 channel, while the red fluorescence can be analyzed by FL2 channel.In addition to flow cytometry, it can also be used in fluorescence imaging and fluorescent plate detection platform.

TD0056

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0.98

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