ELK Biotechnology

Pig ASMA(Anti-Smooth Muscle Antibody) ELISA Kit

Product Code:
 
ELK9257
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Porcine (pig)
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9257-48T48T£261.00
Quantity:
ELK9257-96T96T£335.00
Quantity:
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This product comes from: China.
Typical lead time: 14-21 working days.
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Further Information

Alternative Names:
ASM Ab; Anti Smooth Muscle Antibody
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
7.82-500 ng/mL
Research Area:
Signal transduction
Sample type:
tissue homogenates, cell lysates and other biological fluids
Sensitivity:
2.9 ng/mL
Standard:
500 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anti-Smooth Muscle Antibody(ASMA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-Smooth Muscle Antibody(ASMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anti-Smooth Muscle Antibody(ASMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Smooth Muscle Antibody(ASMA) in the samples is then determined by comparing the OD of the samples to the standard curve.