ELK Biotechnology

Human TSHRAb(Thyroid Stimulating Hormone Receptor Antiboby) ELISA Kit

Product Code:
 
ELK9540
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9540-48T48T£298.00
Quantity:
ELK9540-96T96T£390.00
Quantity:
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Further Information

Alternative Names:
TSHRAb
Assay length:
2h
Assay Type:
Competitive Inhibition
Detection range:
1.57-100mIU/mL
Research Area:
Signal Transduction; Neuroscience
Sample type:
Serum, plasma and other biological fluids
Sensitivity:
0.85 mIU/mL
Standard:
100mIU/mL
Test principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroid Stimulating Hormone Receptor Antiboby(TSHRAb) in the samples is then determined by comparing the OD of the samples to the standard curve.