ELK Biotechnology

Rabbit GFRa1(Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1) ELISA Kit

Product Code:
 
ELK9656
Product Group:
 
ELISA Kits
Supplier:
 
ELK Biotechnology
Regulatory Status:
 
RUO
Target Species:
 
Rabbit
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
 

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CodeSizePrice
ELK9656-48T48T£298.00
Quantity:
ELK9656-96T96T£390.00
Quantity:
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This product comes from: China.
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Further Information

Alternative Names:
GDNFR; GDNFRA; GFR-ALPHA-1; RET1L; RETL1; TRNR1; GDNF family receptor alpha-1; TGF-beta-related neurotrophic factor receptor 1
Assay length:
3.5h
Assay Type:
Sandwich
Detection range:
0.32-20 ng/mL
Research Area:
Infection immunity;
Sample type:
Tissue homogenates and other biological fluids.
Sensitivity:
0.119 ng/mL
Standard:
20 ng/mL
Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1(GFRa1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1(GFRa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1(GFRa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1(GFRa1) in the samples is then determined by comparing the OD of the samples to the standard curve.