Human KDM2B(Lysine (K) specific demethylase 2B) ELISA kit

[Histone-H3]-lysine-36 demethylase 1B; CXXC-type zinc finger protein 2; CXXC2; F box and leucine rich repeat protein 10; F box protein FBL10; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; Fbl10; FBXL10; JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein; JHDM1B; JmjC domain-containing histone demethylation protein 1B; Jumonji C domain containing histone demethylase 1B; Jumonji domain-containing EMSY-interactor methyltransferase motif protein; kdm2b; KDM2B_HUMAN; Lysine (K) specific demethylase 2B; Lysine-specific demethylase 2B; PCCX2;? Protein containing CXXC domain 2; Protein JEMMA; Protein-containing CXXC domain 2;

3.5h

Sandwich

0.32-20 ng/mL

Epigenetics and Nuclear Signaling

serum, plasma and other biological fluids

0.115 ng/mL

20 ng/mL

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysine (K) specific demethylase 2B(KDM2B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lysine (K) specific demethylase 2B(KDM2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysine (K) specific demethylase 2B(KDM2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysine (K) specific demethylase 2B(KDM2B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Q8NHM5