KRIBIOLISA™ Teriparatide (PTH -34) ELISA

0.01 to 1000 ng/ml

Colorimetric, 450nm

Immunoassay for quantitative estimation of Teriparatide in human serum, plasma and tissue extracts. Teriparatide is a recombinant protein form of parathyroid hormone consisting of the first (N-terminus) 34 amino acids, which is the bioactive portion of the hormone. It is an effective anabolic (i.e., bone growing) agent used in the treatment of some forms of osteoporosis. The Teriparatide ELISA kit is designed to measure teriparatide with high specificity while minimizing crossreactivity to human PTH (1-84) and other PTHrPs. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The Teriparatide ELISA is a competitive immunoassay for the determination of Teriparatide. The antiserum is captured by antibodies coated on a 96-well plate. A constant concentration of Biotin Concentrate and varying concentrations of unlabeled standard or sample compete for binding specifically to the antiserum. Captured Bt-tracer is subsequently bound by SA-HRP (streptavidin-conjugated horseradish peroxidase), which produces a soluble colored product after a substrate is added. The enzyme reaction is stopped by dispensing an acidic solution (H2SO4) into the wells after 10 min at room temperature turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is inversely proportional to the amount of specific Teriparatide bound.

Human serum, plasma and tissue extracts

1 ng/ml