KRIBIOLISA™ Anti-Teriparatide ELISA

10 ng/ml - 640 ng/ml

Colorimetric, 450nm

Enzyme Immunoassay for the Quantitative Determination of AntiTeriparatide in serum, plasma and cell culture supernatant. Teriparatide is a recombinant protein form of parathyroid hormone consisting of the first (N-terminus) 34 amino acids, which is the bioactive portion of the hormone. It is an effective anabolic (i.e., bone growing) agent used in the treatment of some forms of osteoporosis. The Teriparatide ELISA kit is designed to measure teriparatide with high specificity while minimizing crossreactivity to human PTH (1-84) and other PTHrPs Krishgen PK kits, developed using the innovator drug as calibrator, are used for the quantitation of serum protein drug levels, and can be used for biosimilar and generics research as well. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year The method employs the quantitative sandwich enzyme immunoassay technique. Teriparatide is pre-coated onto microwells. Samples and standards are pipetted into microwells and antibodies to Teriparatide present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated Teriparatide is pipetted and incubated. After washing microwells in order to remove any nonspecific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Anti-Teriparatide in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Serum and Plasma

10 ng/ml