KRIBIOLISA™ SP 2/0 HCP ELISA

0 ng/ml - 300 ng/ml

Colorimetric, 450nm

Immunoassay for the quantitative estimation of total SP 2/0 Host Cell Proteins. Recombinant expression by the hybridoma cell line SP2/0 is a relatively simple and cost effective method for production of monoclonal antibodies intended for use as therapeutic agents in humans. About the kit: - Uses anti-idiotypic antibodies sourced from our vendor partner in the US, which ensures higher specificity, and low cross reactivity. - Recovery rates are between 85 - 115% - Ready to use with a standard protocol with break-apart pre-coated wells - Validated as per US FDA guidelines for Bioassays - Optimized for matrix effects to ensure higher sensitivity. - Shelf life: 1 year This assay is based on the Sandwich ELISA procedure. Microwells are coated with anti- SP2/0 antibody. Addition of Anti- SP2/0:HRP Conjugate and standards or samples containing SP2/0 HCPs will form a immune complex with the coated anti- SP2/0 antibody in microwell. A washing cycle will remove free Anti- SP2/0:HRP Conjugate from the reaction well. Addition of TMB Substrate will produce blue color in microwells conataining immune complex. Addition of stop solution will terminate the enzymatic reaction and blue color will be converted to yellow color which is directly proportional to the concentration of SP2/0 HCPs present and absorbance value is measured at 450nm.

Cell Culture Supernatant and other Biological Preparations

Please refer to the current IFU for the updated calibrator range and sensitivity.