CircuLex Human ISG15 ELISA Kit
Product Code:
MBL-CY-8085
MBL-CY-8085
Host Type:
Human
Human
Regulatory Status:
RUO
RUO
Application:
Enzyme-Linked Immunosorbent Assay (ELISA)
Enzyme-Linked Immunosorbent Assay (ELISA)
Shipping:
4°C
4°C
Storage:
4°C
4°C
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Code | Size | Price |
---|
MBL-CY-8085 | 96 Assays | £602.00 |
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Typical lead time: 10-14 working days.
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- Further Information
- Documents
- References
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Further Information
Background:
ISG15 (Interferon (IFN)-Stimulated Gene 15) is a ubiquitin-like protein containing two ubiquitin
homology domains and becomes conjugated to a variety of proteins when cells are treated with type I
interferon or lipopolysaccharide. Therefore this modification (so called, ISGylation) appear to be
involved in type I interferon signal transduction. Unlike ubiquitylation, ISGylation does not drive protein
degradation (regulated by K48-linked ubiquitin). ISG15 is expressed as a 165 amino acid precursor that
is subsequently processed to expose the C-terminal sequence LRLRGG. Like ubiquitin, this C-terminal
diglycine residue is adenlylated and conjugated by a thiolester bond to sequential cystine residues on the
E1-activating, E2-carrier and E3-ligase enzymes, before being transferred to lysine residues on target
protein substrates.
ISG15 can also be found extracellularly in an unconjugated form (free form). Human lymphocytes
and monocytes were reported to release free ISG15 following treatment with IFN-β. More than 50 % of
the total ISG15 could be detected in the culture medium after 24 hours following IFN stimulation. In
addition, ISG15 is highly elevated and extensively conjugated to cellular proteins in many tumors and
tumor cell lines. The increased levels of ISG15 in tumor cells were found to be associated with
decreased levels of polyubiquitinated proteins.
Description:
The CycLex Research Product CircuLex Human ISG15 ELISA kit is used for the quantitative
measurement of human ISG15 in cell lysate, culture medium and human serum.
Kit Components:
Microplate, 10X Wash Buffer, Dilution Buffer, Human ISG15 Standard, HRP conjugated Detection Antibody, Substrate Reagent, Stop Solution
Measurement Range:
0.188 ng/mL to 12 ng/ml
Sensitivity:
better than 0.85 ng/ml of sample.
Target:
ISG15
Documents
References
1. Korant BD, Blomstrom DC, Jonak GJ, Knight E, Jr (1984) J Biol Chem; 259: 14835?14839.
2. Blomstrom DC, Fahey D, Kutny R, Korant BD, Knight E Jr (1986) J Biol Chem 261: 8811?8816.
3. Haas AL, Ahrens P, Bright PM, Ankel H (1987) J Biol Chem 1987; 262: 11315?11323.
4. Feltham, N., Hillman, M., Jr., Cordova, B., Fahey, D., Larsen, B., Blomstrom, DC. & Knight, E., Jr.
(1989) J. Interferon Res. 9: 493-507.
5. Knight, E Jr & B. Cordova (1991) J Immunol. 146: 2280-2284.
6. Loeb, K. L. & Haas, A. L. (1992) J. Biol. Chem. 267: 7806-7813.
7. D'Cunha, J, Knight E Jr, Haas A.L., Truitt R. L. & Borden EC. (1996) Proc Natl Acad Sci USA 93:
211-215
8. D'Cunha, J, Ramanujam S., Wagner R.J., Witt PL., Knight E. Jr. & Borden EC. (1996) J Immunol
157: 4100-4108.
9. Austin, K J, S. K. Ward, M. G. Teixeira, Dean VC., Moore DW. & Hansen TR. (1996) Biol Reprod
54: 600-606
10. Kelso A (1998) Immunol Cell Biol 76: 300-317
11. Durfee Larissa A., Lyon Nancy, Seo Kyungwoon, and Huibregtse Jon M. (2010) Mol Cell 38:
722-732.