CircuLex PCSK9 R194A (Human)
Product Code:
MBL-CY-R2333
MBL-CY-R2333
Host Type:
Human
Human
Regulatory Status:
RUO
RUO
Shipping:
Dry Ice
Dry Ice
Storage:
-70°C
-70°C
No additional charges, what you see is what you pay! *
| Code | Size | Price |
|---|
| MBL-CY-R2333 | 20 ug | £578.00 |
Quantity:
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* Rare exceptions are clearly labelled (only 0.14% of items!).
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Typical lead time: 10-14 working days.
Contact us for more accurate information.
- Further Information
- Documents
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Further Information
Background:
PCSK9 binds to the LDL receptor in vitro and in vitro. Human PCSK9 R194A
containing a C-terminal His-tag, expressed in HEK 293 cells. Reduction of binding activity to EGF-AB
domain of LDL receptor, compared to wild type PCSK9, was confirmed by using CircuLex
PCSK9-LDLR in vitro Binding Assay Kit (Cat# CY-8150).
Concentration:
100 ug/mL
Formulation:
The PCSK9 R194A is supplied frozen in PBS pH 7.2, 10 % glycerol.
Gene IDs:
Human: 255738 Mouse: 100102
MW:
The PCSK9 R194A demonstrates 64 kDa (catalytic and C-terminal domain) and 16
kDa (prodomain) by SDS-PAGE analysis.
Purification:
Purified by Ni-chelating chromatography
Purity:
The PCSK9 R194A is greater than 95 % pure as determined by SDS-PAGE analysis.
Shelf Life:
1 year
Source:
Full length human PCSK9 having one mutation R194A, containing C-terminal His-tag,
expressed in HEK 293 cells.
Target:
PCSK9
Documents
References
1. Seidah NG, Benjannet S, Wickham L, Marcinkiewicz J, Jasmin SB, Stifani S, Basak A, Prat A,
Chretien M (2003) Proc Natl Acad Sci USA 100:928?933.
2. Abifadel M, Varret M, Rabes JP, Allard D, Ouguerram K, Devillers M, Cruaud C, Benjannet S,
Wickham L, Erlich D, et al. (2003) Nat Genet 34:154?156.
3. Leren TP (2004) Clin Genet 65:419?422.
4. Allard D, Amsellem S, Abifadel M, Trillard M, Devillers M, Luc G, Krempf M, Reznik Y, Girardet JP,
Fredenrich A, et al. (2005) Hum Mutat 26:497.
5. Cohen JC, Boerwinkle E, Mosley TH, Jr, Hobbs HH (2006) N Engl J Med 354, 1264?1272.
6. Berge KE, Ose L, Leren TP (2006) Arterioscler Thromb Vasc Biol 26:1094?1100.
7. Maxwell KN, Breslow JL (2004) Proc Natl Acad Sci USA 101:7100?7105.
8. Rashid S, Curtis DE, Garuti R, Anderson NN, Bashmakov Y, Ho YK, Hammer RE, Moon YA, Horton
JD (2005) Proc Natl Acad Sci USA 102:5374?5379.
9. Lagace, T. A., Curtis, D. E., Garuti, R., McNutt, M. C., Park, S. W., Prather, H. B., Anderson, N. N.,
Ho, Y. K., Hammer, R. E., and Horton, J. D. (2006) J. Clin. Investig. 116, 2995?3005 10. Cameron, J., Holla, O. L., Ranheim, T., Kulseth, M. A., Berge, K. E., and Leren, T. P. (2006) Hum.
Mol. Genet. 15, 1551?1558
11. Zhang, D. W., Lagace, T. A., Garuti, R., Zhao, Z., McDonald, M., Horton, J. D., Cohen, J. C., and
Hobbs, H. H. (2007) J. Biol. Chem. 282, 18602?18612
12. Hyock Joo Kwon, Thomas A. Lagace, Markey C. McNutt, Jay D. Horton, and Johann Deisenhofer
(2008) ) Proc Natl Acad Sci USA 105: 1820?1825.
13. Matthew J. et al. (2009) J. Biol. Chem. 284, 1313-1323.