Anti-His-tag mAb

WB - 1 ug/mL (chemiluminescence detection system)

A variety of plasmids contain DNA that encodes an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning sites. The His-Tag fusion protein expression system is commonly used because the 6 x His-Tag on the recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography. This specific antibody for His-Tag fusion protein is useful for monitoring of the fusion protein expression and affinity purification.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 57016 Mouse: 15109

6xHis synthetic peptide

This antibody reacts with His-Tag on Western blotting.

1 year

This antibody was purified from hybridoma (clone 6C4) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3U1 with Balb/c mouse splenocyte immunized with synthetic peptide 6 His.

His