Anti-β-galactosidase mAb

WB - 1 ug/mL (chemiluminescence detection system) IP - 1 ug / 200 uL of cell extract from 5x106 cells ICC - 5 ug/mL IHC - 10 ug/mL

β-galactosidase is a homo-tetrameric enzyme, with each subunit having a molecular weight of 116 kDa. Eukaryotic genes are often expressed as fusion protein by the β-galactosidase (lacZ) gene, resulting in the expression of a fusion hybrid with β-galactosidase. Anti-β-galactosidase antibodies provide a simple method to isolate fusion proteins directly from crude bacterial lysates, using immunoaffinity chromatography or immunoprecipitation. Anti-β-galactosidase can also be used for the immunocytochemical detection of β-galactosidase in cells and tissues that express transfected bacterial lacZ gene or β-galactosidase fusion protein.

1 mg/mL

100 ug IgG in 100 ul volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Human: 2720 Mouse: 12091

Length E. coli β-galactosidase

This antibody reacts with β-galactosidase (116kDa) on Western blotting.

1 year

This antibody was purified from hybridoma (clone 5A3) supernatant using protein A agarose. This hybridoma was established by fusion of mouse myeloma cell P3-U1 with Balb/c mouse lymphocyte immunized with E. coli full length β-galactosidase.