MBL

Anti-GST-tag pAb

Product Code:
 
MBL-PM013
Product Group:
 
Primary Antibodies
Supplier:
 
MBL
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
4°C
Storage:
 
-20°C
1 / 2
2 / 2
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MBL-PM013100 ug£269.00
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  • Further Information
  • Documents
  • References
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Further Information

Applications:
WB - 1:1000 IP - 5 uL/sample
Background:
Bacterial Glutathione S-transferase (GST) from Schistosoma japonicum is a widely used protein fusion partner, since it provides exhibits little effect on the biological function of the protein of interest. The GST-tag fusion protein expression system allows for efficient coupling to glutathione (GSH) agarose affinity resins and purification by a single step chromatography. This specific antibody for GST-tag fusion protein is useful for monitoring of the fusion protein expression and affinity purification. It does not recognize mammalian GST.
Formulation:
In PBS/50% glycerol, pH 7.2
Gene IDs:
Human: 373156 Mouse: 56615
Immunogen Translated:
Recombinant GST was purified by column GSH by expressing the pGEX-5X-3 vector
Reactivity:
This antibody reacts with GST on Western blotting.
Shelf Life:
1 year
Source:
This antibody was purified from rabbit serum using protein A agarose. The rabbit was immunized with recombinant bacterial glutathione S-transferase, which was purified from pGEX-5X-3 vector using GSH column.
Target:
GST

Documents

References

1) Sato, M., et al., Int. Immunol. 23, 493-502 (2011) [WB] 2) Zhou, J. and Blissard, G. W., J. Virol. 82, 4449-4460 (2008) [WB] 3) Yamaguchi, T., et al., J. Cell Biol. 171, 431-436 (2005) 4) Yamamoto, H., et al., Mol. Cell Biol.19, 3829-3841 (1999)