Anti-Ribosomal P0/P1/P2 mAb

WB - 0.5-1 ug/mL (chemiluminescence detection system) IP - 1 ug/200 uL of cell extract from 2.0 x 106 cells/sample RIP - 15 ug/500 uL of cell extract from 1.0 x 107 cells/sample Other - Inhibition test (Info. from the licenser. Not tested by MBLI.)

Ribosomal proteins P0 (34 kD), P1 (12 kD), P2 (12 kD) exist as a pentameric complex, P0(P1)2(P2)2 on the large subunits of eukaryotic ribosomes. This complex plays a crucial role in recruitment of translation factors to the ribosome. The P0, P1, P2 components share a common C-terminal amino acid sequence; the sequences of about 20 amino acids at the C-termini are identical among the three proteins. The sequence of the C-terminal ends of P0/P1/P2 is highly conserved among eukaryotes, and is known as the binding site for translation factors. It has also been reported that the C-terminal sequence is an autoimmune target. An autoantibody, called anti-P, from patients with SLE recognizes the conserved C-terminal sequence of P0, P1, and P2. RN004M is a monoclonal antibody reactive with the conserved C-terminal regions of ribosomal P0, P1, and P2. This antibody can therefore be used for wide-range of eukaryotic samples. In addition, anti-P mAb recognizes the native conformation of P0, P1, and P2, and efficiently inhibits protein synthesis (J. Biol. Chem. 265, 89-95 [1990]). This antibody is expected to use for a variety of purposes including studies on ribosome-associated proteins.

1 mg/mL

PBS containing 50% Glycerol (pH 7.2). No preservative is contained.

Human: 617561766181 Mouse: 118375604067186

1 year

Purified IgG from hybridoma supernatant

P0/P1/P2