Elabscience

NAD+/NADH Colorimetric Assay Kit

Product Code:
 
E-BC-K804-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Shipping:
 
Ice packs
Storage:
 
-20°C
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E-BC-K804-M-96T96T£378.00
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Further Information

Assay Time:
70 min
Detection Instrument:
Microplate reader
Detection Range:
0.02-5.0 umol/L
Detection Type:
Colorimetric method
Inter cv:
9.1
Intra cv:
1.8
Result Type:
Quantitative
Sensitivity:
0.02 umol/L
Target Synonym:
NAD+/NADH
Test Principle:
Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.
Tested Sample Types:
animal tissue, cell

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