QuicKey Human CRP(C-Reactive Protein) ELISA Kit

CRP

2.5h

This kit recognizes Human CRP in samples.No significant cross-reactivity or interference between Human CRP and analogues was observed

0.39-25 ng/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

0.23 ng/mL

Human

PTX1

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ? 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

serum, plasma

Sandwich

P02741

This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma.