Elabscience

BCA Protein Colorimetric Assay Kit

Product Code:
 
E-BC-K318-M
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Quantification
Shipping:
 
Ice packs
Storage:
 
RT
1 / 2
2 / 2
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CodeSizePrice
E-BC-K318-M-96T96T£143.00
Quantity:
E-BC-K318-M-500Assays500Assays£177.00
Quantity:
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  • Further Information
  • Documents
  • References
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Further Information

Assay Time:
40 min
Detection Instrument:
Microplate reader
Detection Range:
0.0165-1 mg/mL
Detection Type:
Colorimetric method
Inter cv:
4.5
Intra cv:
2.2
Result Type:
Quantitative
Sensitivity:
0.0165 mg/mL
Target Synonym:
TP
Test Principle:
Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.
Tested Sample Types:
Serum,plasma,cell culture supernatant,tissue,cells

Documents

References

  • https://www.cell.com/immunity/pdf/S1074-7613(23)00025-0.pdf
  • https://www.sciencedirect.com/science/article/pii/S1385894723005685
  • https://www.sciencedirect.com/science/article/pii/S0048969722071790
  • https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-022-01646-9
  • https://www.sciencedirect.com/science/article/pii/S2590006421000971
  • https://www.sciencedirect.com/science/article/pii/S0889159121005067
  • https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.1c02025
  • https://www.mdpi.com/2013838
  • https://www.sciencedirect.com/science/article/pii/S0147651322005929
  • https://www.mdpi.com/1836124
  • https://www.sciencedirect.com/science/article/pii/S0925443923000273
  • https://www.hindawi.com/journals/omcl/2021/9966663/