Elabscience

L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit (Whole Blood Samples)

Product Code:
 
E-BC-K043-S
Product Group:
 
Biochemical Assay Kits
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Quantification
Shipping:
 
Ice packs
Storage:
 
2-8°C
1 / 2
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CodeSizePrice
E-BC-K043-S-50Assays50Assays£224.00
Quantity:
E-BC-K043-S-100Assays100Assays£277.00
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  • Further Information
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Further Information

Assay Time:
60 min
Detection Instrument:
Spectrophotometer
Detection Range:
0.14-7.0 mmol/L
Detection Type:
Colorimetric method
Inter cv:
2
Intra cv:
1.6
Result Type:
Quantitative
Sensitivity:
0.14 mmol/L
Test Principle:
Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.
Tested Sample Types:
Whole blood

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0006295220300848
  • https://biomedical-engineering-online.biomedcentral.com/articles/10.1186/s12938-021-00903-6