Elabscience

Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method)

Product Code:
 
E-BC-K020-M
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
Reagent3:-20°C;Others:2-8°C
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E-BC-K020-M-48T48T£197.00
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E-BC-K020-M-96T96T£251.00
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E-BC-K020-M-500Assays500Assays£512.00
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  • Further Information
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Further Information

Assay Time:
30 min
Detection Instrument:
Microplate reader
Detection Range:
0.2 -14.4 U/mL
Detection Type:
Colorimetric method
Inter cv:
3.7
Intra cv:
2.9
Result Type:
Enzyme Activity
Sensitivity:
0.2 U/mL
Target Synonym:
T-SOD
Test Principle:
The activity of SOD was measured by WST-1 method in this kit and the principles of the WST-1 is as follows. Xanthine Oxidase (XO) can catalyze WST-1 react with O2.- to generate a water-soluble formazan dye. SOD can catalyze the disproportionation of superoxide anions, so the reaction can be inhibited by SOD, and the activity of SOD is negatively correlated with the amount of formazan dye. Therefore, the activity of SOD can be determined by the colorimetric analysis of WST-1 products.
Tested Sample Types:
Serum,plasma,hydrothorax,ascites,urine,cells,tissue

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S2452199X23000154
  • https://pubs.acs.org/doi/abs/10.1021/acsnano.9b09974
  • https://www.sciencedirect.com/science/article/pii/S0304389421020707
  • https://www.sciencedirect.com/science/article/pii/S2090123222001229
  • https://pubs.acs.org/doi/abs/10.1021/acsami.1c14201
  • https://cmbl.biomedcentral.com/articles/10.1186/s11658-023-00416-1
  • https://www.sciencedirect.com/science/article/pii/S004896972105405X
  • https://www.mdpi.com/1983336
  • https://www.mdpi.com/1905716
  • https://www.sciencedirect.com/science/article/pii/S0925400520305839
  • https://www.mdpi.com/article/10.3390/ijms232315400
  • https://www.nature.com/articles/s41419-020-02777-3