Elabscience

Glutathione Peroxidase (GSH-Px) Activity Assay Kit

Product Code:
 
E-BC-K096-S
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
2-8°C
1 / 2
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CodeSizePrice
E-BC-K096-S-50Assays50Assays£197.00
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E-BC-K096-S-100Assays100Assays£251.00
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  • Further Information
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Further Information

Assay Time:
70 min
Detection Instrument:
Spectrophotometer
Detection Range:
12.65-387 U
Detection Type:
Colorimetric method
Inter cv:
9.3
Intra cv:
4.9
Result Type:
Enzyme Activity
Sensitivity:
12.65 U
Target Synonym:
GSH-PX
Test Principle:
Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH-Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
Tested Sample Types:
Serum,plasma,cells,cell culture supernatant,tissue

Documents

References

  • https://www.mdpi.com/article/10.3390/antiox11112264
  • https://www.mdpi.com/2072-6643/14/15/3179
  • https://www.sciencedirect.com/science/article/pii/S0753332221008003
  • https://www.sciencedirect.com/science/article/pii/S0278691522002599
  • https://www.mdpi.com/2076-3921/9/3/250
  • https://www.sciencedirect.com/science/article/pii/S0024320521012170
  • https://www.hindawi.com/journals/omcl/2020/2978340/
  • https://www.sciencedirect.com/science/article/pii/S1001074222000717
  • https://www.mdpi.com/2077-0383/7/10/315
  • https://pubmed.ncbi.nlm.nih.gov/36160397/
  • https://www.mdpi.com/1332228
  • https://www.sciencedirect.com/science/article/pii/S0009279720310875