Elabscience

Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Product Code:
 
E-BC-F038
Supplier:
 
Elabscience
Regulatory Status:
 
RUO
Application:
 
Activity Assay
Shipping:
 
Ice packs
Storage:
 
-20°C
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E-BC-F038-48T48T£251.00
Quantity:
E-BC-F038-96T96T£344.00
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  • Further Information
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Further Information

Assay Time:
75 min
Detection Instrument:
Fluorescence Microplate Reader
Detection Range:
0.01-0.83 U/L
Detection Type:
Fluorescence method
Inter cv:
9.8
Intra cv:
2.3
Result Type:
Enzyme Activity
Sensitivity:
0.01 U/L
Target Synonym:
ALT
Test Principle:
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.
Tested Sample Types:
Serum, plasma, animal tissue

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S1742706120307509
  • https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13332
  • https://pubs.acs.org/doi/abs/10.1021/acsami.9b19518
  • https://www.sciencedirect.com/science/article/pii/S0278691521001575
  • https://www.mdpi.com/2039948
  • https://www.nature.com/articles/s41598-022-21816-x