QuicKey Pro Mouse E3(Estriol)ELISA Kit

E3

1.5h

This kit recognizes Mouse E3 in samples.No significant cross-reactivity or interference between Mouse E3 and analogues was observed

15.63-1000 pg/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

5.83 pg/mL

Mouse

Estriol;E3;

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse E3. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse E3 are added to the micro ELISA plate wells. Mouse E3 in samples (or standards) competes with a fixed amount of E3 on the solid phase supporter for sites on the HRP linked detection antibody specific to E3. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The concentration of Mouse E3 in the samples is then determined by comparing the OD of the samples to the standard curve.

serum, plasma

Competitive

This ELISA kit applies to the in vitro quantitative determination of Mouse E3 concentrations in serum, plasma.