QuicKey Pro Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

IGF-1

1.5h

This kit recognizes Human IGF-1 in samples.No significant cross-reactivity or interference between Human IGF-1 and analogues was observed

3.12-100 ng/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

1.01 ng/mL

Human

insulin-like growth factor 1;IGF1;

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IGF-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IGF-1 are added to the micro ELISA plate wells. Human IGF-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IGF-1.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450?2 nm. The OD value is proportional to the concentration of Human IGF-1. The concentration of Human IGF-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

serum, plasma

Sandwich

P05019

This ELISA kit applies to the in vitro quantitative determination of Human IGF-1 concentrations in serum, plasma.