Elabscience

p21 Polyclonal Antibody

Product Code:
 
E-AB-66357
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of 293T cells using p21 Polyclonal Antibody at dilution of 1:1000.
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Immunofluorescence analysis of NIH/3T3 cells using p21 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of 293T cells using p21 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of NIH/3T3 cells using p21 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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E-AB-66357-60uL60uL£210.00
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E-AB-66357-120uL120uL£291.00
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E-AB-66357-200uL200uL£431.00
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  • Further Information
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Further Information

Abbreviation:
p21
Background:
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Calculated MW:
21kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000 IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human p21 (NP_000380.1).
ObservedMW:
21kDa
Purification method:
Affinity purification
Target Synonym:
CAP20;CDKN1;CIP1;MDA-6;P21;SDI1;WAF1;p21CIP1;CDKN1A
UNIProt ID:
P38936

Documents

References

  • https://www.nature.com/articles/s41467-022-28721-x
  • https://www.mdpi.com/1574002
  • https://www.future-science.com/doi/abs/10.2144/btn-2022-0046