Elabscience

APC Anti-Mouse F4/80 Antibody[CI:A3-1]

Product Code:
 
E-AB-F0995UE
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rat
Antibody Isotype:
 
Rat IgG2b, κ
Antibody Clone:
 
CI:A3-1
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Flow Cytometry
Shipping:
 
Ice packs
Storage:
 
Store at 2-8°C and protected from prolonged exposure to light. Do not freeze.
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C57BL/6 murine abdominal macrophages are stained with APC Anti-Mouse F4/80 Antibody (filled gray histogram). Unstained abdominal macrophages (empty black histogram) are used as control.
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C57BL/6 murine abdominal macrophages are stained with APC Anti-Mouse F4/80 Antibody (filled gray histogram). Unstained abdominal macrophages (empty black histogram) are used as control.

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CodeSizePrice
E-AB-F0995UE-25ug25ug£103.00
Quantity:
E-AB-F0995UE-100ug100ug£157.00
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Further Information

Abbreviation:
F4/80
Background:
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.
Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Concentration:
0.2 mg/mL
Conjugation:
APC
GeneID:
13733
Target Synonym:
Adhesion G protein-coupled receptor E1;Adgre1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Adgre1;Emr1; Gpf480;
UNIProt ID:
Q61549
Usage:
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 ug/10^6 cells in 100 uL volume].

Documents

References

  • https://www.sciencedirect.com/science/article/pii/S0014299922005325
  • https://link.springer.com/article/10.1007/s12011-022-03362-9