25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit

25-HVD3

2.5h

This kit recognizes Universal 25-HVD3 in samples.No significant cross-reactivity or interference between Universal 25-HVD3 and analogues was observed

3.13-200 ng/mL

Colormetric

Both intra-CV and inter-CV are < 10%.

80%-120%

1.88 ng/mL

Universal

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 25-HVD3. During the reaction, Universal 25-HVD3 in the sample or standard competes with a fixed amount of Universal 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 25-HVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ? 2 nm. The concentration of Universal 25-HVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

serum, plasma and other biological fluids

Competitive

This ELISA kit applies to the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma and other biological fluids.