Elabscience

NCL Polyclonal Antibody

Product Code:
 
E-AB-68385
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines using NCL Polyclonal Antibody at 1:1000 dilution.
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Immunohistochemistry of paraffin-embedded human liver cancer using NCL Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using NCL Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver cancer using NCL Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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CodeSizePrice
E-AB-68385-60uL60uL£210.00
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E-AB-68385-120uL120uL£291.00
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E-AB-68385-200uL200uL£431.00
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Further Information

Abbreviation:
NCL
Background:
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
76kDa
Concentration:
1 mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
Recombinant fusion protein of human NCL
ObservedMW:
110kDa
Purification method:
Affinity purification
Target Synonym:
NCL;C23;Nsr1;nucleolin
UNIProt ID:
P19338

Documents