Elabscience

Rad23B Polyclonal Antibody

Product Code:
 
E-AB-92397
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Immunohistochemistry of paraffin-embedded human colon using Rad23B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunofluorescence analysis of U2OS cells using Rad23B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human colon using Rad23B Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using Rad23B Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

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E-AB-92397-60uL60uL£210.00
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E-AB-92397-120uL120uL£291.00
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E-AB-92397-200uL200uL£431.00
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Further Information

Abbreviation:
Rad23B
Background:
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Buffer:
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Calculated MW:
35kDa/43kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
A synthetic peptide of human Rad23B
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
RAD23B; HHR23B; HR23B; P58; UV excision repair protein RAD23 homolog B
UNIProt ID:
P54727

Documents