Elabscience

PCIF1 Polyclonal Antibody

Product Code:
 
E-AB-92399
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Immunohistochemistry of paraffin-embedded rat brain using PCIF1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug PCIF1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PCIF1 Polyclonal Antibody at a dilution of 1:1000.
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Immunohistochemistry of paraffin-embedded rat brain using PCIF1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug PCIF1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PCIF1 Polyclonal Antibody at a dilution of 1:1000.

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E-AB-92399-60uL60uL£210.00
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E-AB-92399-120uL120uL£291.00
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E-AB-92399-200uL200uL£431.00
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Further Information

Abbreviation:
PCIF1
Background:
Cap-specific adenosine methyltransferase that catalyzes formation of N(6,2'-O-dimethyladenosine cap (m6A(m by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII via interaction with POLR2A and mediates formation of m6A(m co-transcriptionally.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
80kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IP 1:50-1:200
Immunogen:
Recombinant fusion protein of human PCIF1
ObservedMW:
Refer to figures
Purification method:
Affinity purification
Target Synonym:
PCIF1; C20orf67; PPP1R121; PDX1 C-terminal inhibiting factor 1
UNIProt ID:
Q9H4Z3

Documents