Elabscience

RAB35 Polyclonal Antibody

Product Code:
 
E-AB-92824
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 2
Western blot analysis of extracts of HeLa cells using RAB35 Polyclonal Antibody at 1:1000 dilution.
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Immunofluorescence analysis of PC-12 cells using RAB35 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of HeLa cells using RAB35 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of PC-12 cells using RAB35 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-92824-60uL60uL£210.00
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E-AB-92824-120uL120uL£291.00
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E-AB-92824-200uL200uL£431.00
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Further Information

Abbreviation:
RAB35
Background:
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2 and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
17kDa/23kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
A synthetic peptide of human RAB35
ObservedMW:
23KDa
Purification method:
Affinity purification
Target Synonym:
RAB35;H-ray;RAB1C;RAY
UNIProt ID:
Q15286

Documents