Elabscience

LEO1 Polyclonal Antibody

Product Code:
 
E-AB-92916
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines using LEO1 Polyclonal Antibody at 1:1000 dilution.
2 / 4
Immunohistochemistry of paraffin-embedded human esophageal cancer using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 4
Immunohistochemistry of paraffin-embedded mouse liver using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 4
Immunofluorescence analysis of U2OS cells using LEO1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using LEO1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human esophageal cancer using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse liver using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using LEO1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-92916-60uL60uL£210.00
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E-AB-92916-120uL120uL£291.00
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E-AB-92916-200uL200uL£431.00
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Further Information

Abbreviation:
LEO1
Background:
LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
68kDa/75kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000,IHC 1:50-1:200,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human LEO1
ObservedMW:
105KDa
Purification method:
Affinity purification
Target Synonym:
LEO1;RDL
UNIProt ID:
Q8WVC0

Documents