Elabscience

METTL14 Polyclonal Antibody

Product Code:
 
E-AB-93402
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines using METTL14 Polyclonal Antibody at 1:500 dilution.
2 / 5
Immunohistochemistry of paraffin-embedded human colon using METTL14 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
3 / 5
Immunohistochemistry of paraffin-embedded mouse testis using METTL14 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 5
Immunofluorescence analysis of U2OS cells using METTL14 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug METTL14 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using METTL14 Polyclonal Antibody at a dilution of 1:500.
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Western blot analysis of extracts of various cell lines using METTL14 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded human colon using METTL14 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse testis using METTL14 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using METTL14 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug METTL14 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using METTL14 Polyclonal Antibody at a dilution of 1:500.

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E-AB-93402-60uL60uL£210.00
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E-AB-93402-120uL120uL£291.00
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E-AB-93402-200uL200uL£431.00
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This product comes from: China.
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Further Information

Abbreviation:
METTL14
Background:
The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis. In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core. N6-methyladenosine (m6A, which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing. M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity. In embryonic stem cells (ESCs, m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
52kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200,IP 1:50-1:200
Immunogen:
Recombinant fusion protein of human METTL14
ObservedMW:
65KDa
Purification method:
Affinity purification
Target Synonym:
METTL14;hMETTL14
UNIProt ID:
Q9HCE5

Documents